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high pure mirna isolation kit  (Roche)


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    Roche high pure mirna isolation kit
    High Pure Mirna Isolation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+pure+mirna+isolation+kit/nct02448056-42-4-9
    Average 86 stars, based on 1 article reviews
    high pure mirna isolation kit - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Isolation:

    Article Title: MiRNA as a Diagnostic and Prognostic Biomarker of Hepatocellular Carcinoma
    Article Snippet: .. RNA will isolated using High Pure miRNA Isolation Kit (Roche, Mannheim, Germany) according to the manufacturer's instructions and then stored at -80 _C until the experiment. .. Reverse transcription reaction For miRNA analyses by quantitative real-time polymerase chain reaction (qRT-PCR), 100 ng of total RNA will be reverse-transcribed using the TaqMan miRNA Reverse Transcription Kit (Applied Biosystems, Carlsbad, CA, USA).

    Article Title: Methods for diagnosing and/or treating acute or chronic liver, kidney or lung disease
    Article Snippet: .. Commercial kits for mRNA purification are also available, including, without limitation, miRNeasy Mini Kit from Qiagen, miRNA Life Technologies isolation kits, mirPremier the microRNA isolation kit from Sigma-Aldrich and High Pure miRNA Isolation Kit from Roche. .. In a particular embodiment the integrity of the RNA was analyzed using RNA 6000 Nano Chips in the Agilent 2100 bionalizer (Agilent Technologies, Palo Alto, CA, USA).

    Article Title: Expansion of the sagittal suture induces proliferation of skeletal stem cells and sustains endogenous calvarial bone regeneration.
    Article Snippet: .. After culturing, cells were dissociated from respective plates, and RNA was immediately isolated with the High Pure miRNA Isolation Kit (Roche, Basel, Switzerland) per manufacturer instructions. qPCR reactions were carried out using the TaqMan gene expression analysis kit. ..

    Article Title: Experimentally altering microRNA levels in embryos alters adult phenotypes
    Article Snippet: .. RNA was isolated using a High Pure miRNA Isolation Kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions and stored at − 80 °C until use. ..

    Article Title: Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency.
    Article Snippet: .. After overnight incubation, cells were washed with PBS and microRNAs were isolated using a High Pure miRNA isolation Kit (Roche, Prague, Czech Republic) according to the manufacturer’s instructions20. .. The yield of microRNA was determined using Invitrogen Qubit RNA high sensitivity (HS) Kit (Thermo Fisher Scientific, Prague, Czech Republic). cDNA synthesis.

    Article Title: A systematic review of non-coding RNA genes with differential expression profiles associated with autism spectrum disorders
    Article Snippet: .. Ozkul et al., 2020 [ ] , Blood: serum , Yes , Not stated , 31:14 , 2–13 = range , 10:11 , 3–16 = range , , , High Pure miRNA Isolation Kit (Roche) , , , 372 miRNA genes (384HC miScript miRNA PCR Array) using TaqMan Universal PCR Master Mix with qPCR on BioMark system (Fluidigm) , 2 –ΔΔCt , SNORD61 , SNORD68 , SNORD72 , SNORD95 , SNORD96A , RNU6-2 , miRTC , and PPC. .. Pagan et al., 2017 [ ] , Post-mortem pineal gland , Yes , Yes , 8:1 , 39 = median , 20:2 , 36.5 = median , mirVana PARIS kit (Ambion) , Single miRNA gene examined by qPCR (machine not specified) using miScript reverse transcription kit and SYBR Green PCR kit (Qiagen) , 2 –ΔΔCt P < 0.0001 , Spiked in cel-miR39 , cel-miR54 and cel-miR238 (Qiagen).

    Article Title: Syringin Prevents 6-Hydroxydopamine Neurotoxicity by Mediating the MiR-34a/SIRT1/Beclin-1 Pathway and Activating Autophagy in SH-SY5Y Cells and the Caenorhabditis elegans Model.
    Article Snippet: Subsequently, the acetylation of Beclin-1 was determined via Western blotting using the Acet K monoclonal antibody (Cell Signaling Technology). .. According to the manufacturer’s instructions, miRNAs were isolated using the High Pure miRNA Isolation Kit (Roche Diagnostics, GmbH, Mannheim, Germany), and the concentration of the extracted RNA was calculated using an ultraviolet spectrophotometer. miRNA was reverse transcribed using the MystiCq microRNA cDNA Synthesis Mix kit (Sigma–Aldrich). .. Finally, we used the MystiCq microRNA qPCR Assay System (Sigma– Aldrich) and the ABI StepOnePlus system (Applied Biosystems, Inc., Foster City, CA, USA) to detect the miR-34a expression.

    Article Title: Bioproduced Nanoparticles Deliver Multiple Cargoes via Targeted Tumor Therapy In Vivo.
    Article Snippet: .. The microRNA was isolated using High Pure miRNA Isolation Kit (Roche, Prague, Czech Republic),23 and its amount was determined using Invitrogen Qubit RNA High Sensitivity Kit (Thermo Fisher Scientific, Prague, Czech Republic). .. High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Prague, Czech Republic) was applied for cDNA synthesis.

    Purification:

    Article Title: Methods for diagnosing and/or treating acute or chronic liver, kidney or lung disease
    Article Snippet: .. Commercial kits for mRNA purification are also available, including, without limitation, miRNeasy Mini Kit from Qiagen, miRNA Life Technologies isolation kits, mirPremier the microRNA isolation kit from Sigma-Aldrich and High Pure miRNA Isolation Kit from Roche. .. In a particular embodiment the integrity of the RNA was analyzed using RNA 6000 Nano Chips in the Agilent 2100 bionalizer (Agilent Technologies, Palo Alto, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Expansion of the sagittal suture induces proliferation of skeletal stem cells and sustains endogenous calvarial bone regeneration.
    Article Snippet: .. After culturing, cells were dissociated from respective plates, and RNA was immediately isolated with the High Pure miRNA Isolation Kit (Roche, Basel, Switzerland) per manufacturer instructions. qPCR reactions were carried out using the TaqMan gene expression analysis kit. ..

    Article Title: A systematic review of non-coding RNA genes with differential expression profiles associated with autism spectrum disorders
    Article Snippet: .. Ozkul et al., 2020 [ ] , Blood: serum , Yes , Not stated , 31:14 , 2–13 = range , 10:11 , 3–16 = range , , , High Pure miRNA Isolation Kit (Roche) , , , 372 miRNA genes (384HC miScript miRNA PCR Array) using TaqMan Universal PCR Master Mix with qPCR on BioMark system (Fluidigm) , 2 –ΔΔCt , SNORD61 , SNORD68 , SNORD72 , SNORD95 , SNORD96A , RNU6-2 , miRTC , and PPC. .. Pagan et al., 2017 [ ] , Post-mortem pineal gland , Yes , Yes , 8:1 , 39 = median , 20:2 , 36.5 = median , mirVana PARIS kit (Ambion) , Single miRNA gene examined by qPCR (machine not specified) using miScript reverse transcription kit and SYBR Green PCR kit (Qiagen) , 2 –ΔΔCt P < 0.0001 , Spiked in cel-miR39 , cel-miR54 and cel-miR238 (Qiagen).

    Gene Expression:

    Article Title: Expansion of the sagittal suture induces proliferation of skeletal stem cells and sustains endogenous calvarial bone regeneration.
    Article Snippet: .. After culturing, cells were dissociated from respective plates, and RNA was immediately isolated with the High Pure miRNA Isolation Kit (Roche, Basel, Switzerland) per manufacturer instructions. qPCR reactions were carried out using the TaqMan gene expression analysis kit. ..

    Incubation:

    Article Title: Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency.
    Article Snippet: .. After overnight incubation, cells were washed with PBS and microRNAs were isolated using a High Pure miRNA isolation Kit (Roche, Prague, Czech Republic) according to the manufacturer’s instructions20. .. The yield of microRNA was determined using Invitrogen Qubit RNA high sensitivity (HS) Kit (Thermo Fisher Scientific, Prague, Czech Republic). cDNA synthesis.

    Polymerase Chain Reaction:

    Article Title: A systematic review of non-coding RNA genes with differential expression profiles associated with autism spectrum disorders
    Article Snippet: .. Ozkul et al., 2020 [ ] , Blood: serum , Yes , Not stated , 31:14 , 2–13 = range , 10:11 , 3–16 = range , , , High Pure miRNA Isolation Kit (Roche) , , , 372 miRNA genes (384HC miScript miRNA PCR Array) using TaqMan Universal PCR Master Mix with qPCR on BioMark system (Fluidigm) , 2 –ΔΔCt , SNORD61 , SNORD68 , SNORD72 , SNORD95 , SNORD96A , RNU6-2 , miRTC , and PPC. .. Pagan et al., 2017 [ ] , Post-mortem pineal gland , Yes , Yes , 8:1 , 39 = median , 20:2 , 36.5 = median , mirVana PARIS kit (Ambion) , Single miRNA gene examined by qPCR (machine not specified) using miScript reverse transcription kit and SYBR Green PCR kit (Qiagen) , 2 –ΔΔCt P < 0.0001 , Spiked in cel-miR39 , cel-miR54 and cel-miR238 (Qiagen).

    Concentration Assay:

    Article Title: Syringin Prevents 6-Hydroxydopamine Neurotoxicity by Mediating the MiR-34a/SIRT1/Beclin-1 Pathway and Activating Autophagy in SH-SY5Y Cells and the Caenorhabditis elegans Model.
    Article Snippet: Subsequently, the acetylation of Beclin-1 was determined via Western blotting using the Acet K monoclonal antibody (Cell Signaling Technology). .. According to the manufacturer’s instructions, miRNAs were isolated using the High Pure miRNA Isolation Kit (Roche Diagnostics, GmbH, Mannheim, Germany), and the concentration of the extracted RNA was calculated using an ultraviolet spectrophotometer. miRNA was reverse transcribed using the MystiCq microRNA cDNA Synthesis Mix kit (Sigma–Aldrich). .. Finally, we used the MystiCq microRNA qPCR Assay System (Sigma– Aldrich) and the ABI StepOnePlus system (Applied Biosystems, Inc., Foster City, CA, USA) to detect the miR-34a expression.

    Spectrophotometry:

    Article Title: Syringin Prevents 6-Hydroxydopamine Neurotoxicity by Mediating the MiR-34a/SIRT1/Beclin-1 Pathway and Activating Autophagy in SH-SY5Y Cells and the Caenorhabditis elegans Model.
    Article Snippet: Subsequently, the acetylation of Beclin-1 was determined via Western blotting using the Acet K monoclonal antibody (Cell Signaling Technology). .. According to the manufacturer’s instructions, miRNAs were isolated using the High Pure miRNA Isolation Kit (Roche Diagnostics, GmbH, Mannheim, Germany), and the concentration of the extracted RNA was calculated using an ultraviolet spectrophotometer. miRNA was reverse transcribed using the MystiCq microRNA cDNA Synthesis Mix kit (Sigma–Aldrich). .. Finally, we used the MystiCq microRNA qPCR Assay System (Sigma– Aldrich) and the ABI StepOnePlus system (Applied Biosystems, Inc., Foster City, CA, USA) to detect the miR-34a expression.

    Reverse Transcription:

    Article Title: Syringin Prevents 6-Hydroxydopamine Neurotoxicity by Mediating the MiR-34a/SIRT1/Beclin-1 Pathway and Activating Autophagy in SH-SY5Y Cells and the Caenorhabditis elegans Model.
    Article Snippet: Subsequently, the acetylation of Beclin-1 was determined via Western blotting using the Acet K monoclonal antibody (Cell Signaling Technology). .. According to the manufacturer’s instructions, miRNAs were isolated using the High Pure miRNA Isolation Kit (Roche Diagnostics, GmbH, Mannheim, Germany), and the concentration of the extracted RNA was calculated using an ultraviolet spectrophotometer. miRNA was reverse transcribed using the MystiCq microRNA cDNA Synthesis Mix kit (Sigma–Aldrich). .. Finally, we used the MystiCq microRNA qPCR Assay System (Sigma– Aldrich) and the ABI StepOnePlus system (Applied Biosystems, Inc., Foster City, CA, USA) to detect the miR-34a expression.

    cDNA Synthesis:

    Article Title: Syringin Prevents 6-Hydroxydopamine Neurotoxicity by Mediating the MiR-34a/SIRT1/Beclin-1 Pathway and Activating Autophagy in SH-SY5Y Cells and the Caenorhabditis elegans Model.
    Article Snippet: Subsequently, the acetylation of Beclin-1 was determined via Western blotting using the Acet K monoclonal antibody (Cell Signaling Technology). .. According to the manufacturer’s instructions, miRNAs were isolated using the High Pure miRNA Isolation Kit (Roche Diagnostics, GmbH, Mannheim, Germany), and the concentration of the extracted RNA was calculated using an ultraviolet spectrophotometer. miRNA was reverse transcribed using the MystiCq microRNA cDNA Synthesis Mix kit (Sigma–Aldrich). .. Finally, we used the MystiCq microRNA qPCR Assay System (Sigma– Aldrich) and the ABI StepOnePlus system (Applied Biosystems, Inc., Foster City, CA, USA) to detect the miR-34a expression.



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    Fig. 2. HeatMap and cluster patterns from gene expression pathways affected by <t>miRNAs.</t> A: Target of upregulated miRNAs in placental tissue, placenta-derived cells (Mononuclear cells), peripheral blood, plasma, plasma Evs, and serum B: Target of downregulated miRNAs in placental tissue and placenta-derived exosomes.
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    Fig. 2. HeatMap and cluster patterns from gene expression pathways affected by <t>miRNAs.</t> A: Target of upregulated miRNAs in placental tissue, placenta-derived cells (Mononuclear cells), peripheral blood, plasma, plasma Evs, and serum B: Target of downregulated miRNAs in placental tissue and placenta-derived exosomes.
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    Image Search Results


    Fig. 2. HeatMap and cluster patterns from gene expression pathways affected by miRNAs. A: Target of upregulated miRNAs in placental tissue, placenta-derived cells (Mononuclear cells), peripheral blood, plasma, plasma Evs, and serum B: Target of downregulated miRNAs in placental tissue and placenta-derived exosomes.

    Journal: Obesity Medicine

    Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review

    doi: 10.1016/j.obmed.2023.100487

    Figure Lengend Snippet: Fig. 2. HeatMap and cluster patterns from gene expression pathways affected by miRNAs. A: Target of upregulated miRNAs in placental tissue, placenta-derived cells (Mononuclear cells), peripheral blood, plasma, plasma Evs, and serum B: Target of downregulated miRNAs in placental tissue and placenta-derived exosomes.

    Article Snippet: A rboleda Borrero et al. ObesityMedicine39(2023)100487 19 Table 2 (continued ) SAMPLES AUTHOR RNA EXTRACTION KIT REVERSE TRANSCRIPTION KIT QUANTIFICATION METHOD REFERENCE MOLECULE MICROARRAYS RNA-SEQUENCING System (Applied Biosystems Inc.) Biosystems Inc, CA, USA) Plasma Balci et al., 2020** High Pure miRNA Isolation Kit (Roche) Reverse transcription and pre-amplification (TaqMan, PreAmp Master Mix 29) Dynamic Array Integrated Fluidic Circuits (IFCs). n/a n/a n/a Cao et al., 2017** RNeasy plus mini kit (Qiagen) PrimeScript RT reagent Kit (TaKaRa, Dalian, China) TaqMan miRNA assays U6 n/a n/a Légaré 2022ᵻ** MirVana PARIS kit (Thermo Fisher Scientific) n/a n/a n/a n/a TruSeq Small RNA Sample Prep kit (Illumina, BC, Canada) Peng et al., 2018* Trizol (Invitrogen, Carlsbad, CA, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing, China) β-actin n/a n/a Sebastiani et al., 2017** MiRNeasy miRNA extraction kit (Qiagen, Hilden, Germany) Megaplex RT primers Pool-A (Lifetechnologies, CA, USA) TaqMan miRNA Human Array Panel A platform (Lifetechnologies, CA, USA) and TaqMan miRNA assay primers (Lifetechnologies, CA, USA) miR-320 - miR374a n/a n/a Tagoma et al., 2018** miRNeasy Serum/Plasma Kit (Qiagen, Germany) miScript II RT Kit (Qiagen) miScript SYBR Green PCR Kit (Qiagen) C. elegans miR-39 n/a n/a Wander et al., 2017** Exiqon miRCURYTM RNA Biofluids Isolation Kit (Exiqon, Woburn, MA) n/a RT-qPCR miR-423-3p n/a n/a Wang F 2021ɨ** miRNeasy Serum/Plasma Kit (Qiagen) miScript II RT (Qiagen) miScript SYBR Green PCR Kit (Qiagen) cel-miR-39 SurePrint human miRNA microarray (Agilent Technologies, Santa Clara, CA, USA, G4872A) Yoffe 2019ɨ** miRNeasy Serum/Plasma Kit n/a TaqManTM Advanced miRNA Assay (Applied Biosystems; Thermo Fisher Scientific, Inc.) cel-miR-39 multiplexed NanoString nCounter miRNA expression assay (NanoString Technologies, Seattle, USA) n/a Zhu 2015ᵻ** miRNeasy Serum/Plasma Kit (Qiagen, Hilden, Germany) n/a SYBR Premix Ex Taq II polymerase (TaKaRa, Dalian, China) miR-221 n/a Ion Total RNASeq Kit v2 (Life Technologies, Grand Island, NY, USA)/Ion Torrent (Life Technologies, Grand Island, NY, USA) Peripheral blood Bian et al., 2022* TRIzol® reagent (TaKaRa) Reverse transcriptase (Vazyme) SYBRTM Green PCR Kit (Vazyme) GAPDH and U6 n/a n/a Feng et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems, Carlsbad, CA) RT-qPCR n/a n/a n/a (continued on next page) A .M .

    Techniques: Gene Expression, Derivative Assay, Clinical Proteomics